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Where `MySortedBamFile.bam` is an index/sorted bam file that can be obtained by first mapping the fastq files using an aligner (such as bowtie) and then sorting and indexing using samtools. We recommend the --Correct-PCR option unless you are fairly certain that PCR amplification bias wont be an issue. This option does increase runtime however.
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Where `MySortedBamFile.bam` is an index/sorted bam file that can be obtained by first mapping the fastq files using an aligner (such as bowtie) and then sorting and indexing using samtools.
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