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docs/source/live_cell/live_cell.rst

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.. _livecellimaging-home:
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Live-Cell Imaging
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- Heating pad to wrap around detection objective port
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Live-Cell Imaging Full Assembly
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**Figure 2:** Thermocouple adapter assembly process
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Heater Adapter Assembly
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**Figure 3:** Heater adapter assembly process
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Placing O-rings in the Sample Chamber
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In order to ensure a watertight seal around our objectives, both of our objective ports feature two sets of o-rings
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surrounding their circumference. For our smaller port associated with the TL20X-MPL objective, we use oil-resistant
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**Figure 4:** Preparation and Placement of O-rings in the Sample Chamber
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Placement of the Heating Pads on the Sample Chamber
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The placement of heating pads on the chamber is a straightforward process, particularly with the adhesive-backed pads
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we detailed in our parts list. In the application process, a general consideration should be made as to the
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**Figure 5:** Preparation and Placement of Heating Pads on the Sample Chamber
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Temperature Controller Assembly and Settings
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With the individual components of our heating assembly prepared, the full assembly of the system is straightforward.
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There is some variation in terms of if one opts to use 3 threaded thermocouples or a combination of one dual-probe

docs/source/physical_assembly/physical_assembly.rst

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Ordering Custom Parts from Xometry
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We utilize a number of different custom fabricated elements in our completed system, . There are a number of
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different companies available for this, and we opted for Xometry. The basic ordering process we go through is as

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