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Live-Cell Imaging
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______________________________
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@@ -85,10 +85,10 @@ assembly based on the following components:
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- Heating pad to wrap around detection objective port
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Live-Cell Imaging Full Assembly
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______________________________
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@@ -114,7 +114,7 @@ thermocouple wires:
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**Figure 2: ** Thermocouple adapter assembly process
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+ ------------------------------
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Heater Adapter Assembly
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**Figure 3: ** Heater adapter assembly process
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Placing O-rings in the Sample Chamber
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In order to ensure a watertight seal around our objectives, both of our objective ports feature two sets of o-rings
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surrounding their circumference. For our smaller port associated with the TL20X-MPL objective, we use oil-resistant
@@ -165,10 +165,10 @@ grooves are first coated with vacuum grease in the following process:
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**Figure 4: ** Preparation and Placement of O-rings in the Sample Chamber
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Placement of the Heating Pads on the Sample Chamber
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The placement of heating pads on the chamber is a straightforward process, particularly with the adhesive-backed pads
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we detailed in our parts list. In the application process, a general consideration should be made as to the
@@ -191,10 +191,10 @@ to be as efficient as possible with them.The general assembly process is as foll
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**Figure 5: ** Preparation and Placement of Heating Pads on the Sample Chamber
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Temperature Controller Assembly and Settings
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With the individual components of our heating assembly prepared, the full assembly of the system is straightforward.
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There is some variation in terms of if one opts to use 3 threaded thermocouples or a combination of one dual-probe
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