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Hi,
I followed the tutorial to make a heatmap with Monocle3 pseudotime data.
I have a problem, on the side the curves go up or down but each line of the heatmap has a plain color.
Checking the matrix I see that each row has almost identical values for all columns.
I should point out that my cell_dataset_object
used to make the matrix with pre_pseudotime_matrix
comes from a conversion of a seurat dataset with cds <- as.cell_data_set(scanorama.harmony, reduction="umap" )
, so I took the tuto from the cluster_cells step.
I don't know if the number of cells has something to do with it (I have 969 cells on the subset I want to analyze).
How can I do to get the expected rendering ?
Thanks in advance for your help.
Justine
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