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README.md

Mosquito Midgut Microbiota Analysis

This project analyzes the bacterial communities in the midguts of Culex quinquefasciatus mosquitoes following different blood meal types. The analysis includes visualizations of bacterial abundance changes and species distribution across various conditions.

Project Overview

  • Figure 1: Stacked bar plot showing the relative abundance of bacterial species across individual mosquito midgut samples, grouped by blood meal type and controls.
  • Figure 2: Heatmap illustrating log2 fold changes in bacterial abundance, highlighting species-specific responses to different blood meals.
  • Figure 3: Heatmap showing the percentage distribution of bacterial species, emphasizing the impact of blood source on microbiota composition.

Setup Instructions

  1. Clone the Repository:
    git clone https://github.com/yourusername/your-repo-name.git
    cd your-repo-name
    
    
    

##Install Dependencies: Ensure you have Python and the required libraries installed:

pip install numpy pandas matplotlib seaborn

##Prepare Data: Place your abundance_table_species1.tsv file in the project directory.

##Usage Run the analysis script to generate the visualizations:

python analysis_script.py

##Data Input: abundance_table_species1.tsv - Contains abundance data for bacterial species in mosquito midguts. Output: Visualizations showing species abundance and distribution.

License This project is licensed under the MIT License - see the LICENSE file for details.

Key Points:

  • Project Overview: Briefly describes the purpose and visualizations.
  • Setup Instructions: Guides users on how to clone the repository and install dependencies.
  • Usage: Provides a command to run the analysis.
  • Data: Describes the input and output of the analysis.
  • License and Acknowledgments: Standard sections for open-source projects.

Feel free to modify any sections to better fit your project's specifics or add additional details as needed!

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Heatmap and bar graphs for 16s analysis

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